刘伟志;洪伟;李金龙;

• 1:辽宁中医药大学附属第三医院大肠外科
• 2:辽宁中医药大学附属第三医院电检科

摘要(Abstract)：

目的研究灵芝多糖在结直肠癌细胞中的调控作用。方法以结直肠癌细胞T84和DiFi作为研究对象,并以空白培养基和结直肠癌化疗药物卡培他滨分别作为空白对照和阳性对照。采用MTT法检测灵芝多糖(GLP)对T84和DiFi细胞增殖的影响,划痕愈合实验检测GLP对T84和DiFi细胞迁移能力的影响,Transwell实验检测GLP对T84和DiFi细胞侵袭能力的影响,流式细胞术检测GLP对T84和DiFi细胞凋亡率的影响。结果 MTT实验结果显示,灵芝多糖处理后,结直肠癌细胞的活力和增殖能力均显著低于空白组(P<0.05);划痕愈合实验和Transwell实验结果显示,灵芝多糖处理后,结直肠癌细胞的迁移、侵袭能力均显著低于空白组(P<0.05);流式细胞术检测细胞凋亡结果显示,灵芝多糖处理后结直肠癌细胞凋亡率显著高于空白组(P<0.05);GLP组与卡培他滨组差异无统计学意义(P>0.05)。结论灵芝多糖对结直肠癌细胞的增殖、迁移和侵袭能力均有良好的抑制作用,可促进结直肠癌细胞凋亡。

关键词(KeyWords)： 灵芝多糖;结直肠癌;卡培他滨

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 刘伟志;洪伟;李金龙;

Email:

DOI: 10.14053/j.cnki.ppcr.202108001

参考文献(References)：

• [1] Araghi M,Arnold M,Rutherford MJ,et al.Colon and rectal cancer survival in seven high-income countries 2010-2014:variation by age and stage at diagnosis (the ICBP SURVMARK-2 project)[J].Gut,2021,70(1):114-126.
• [2] Bray F,Ferlay J,Soerjomataram I,et al.Global cancer statistics 2018:GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J].CA Cancer J Clin,2018,68(6):394-424.
• [3] Siegel RL,Miller KD,Jemal A.Cancer statistics,2019[J].CA Cancer J Clin,2019,69(1):7-34.
• [4] Ferlay J,Colombet M,Soerjomataram I,et al.Estimating the global cancer incidence and mortality in 2018:GLOBOCAN sources and methods[J].Int J Cancer,2019,144(8):1941-1953.
• [5] Feng RM,Zong YN,Cao SM,et al.Current cancer situation in China:good or bad news from the 2018 Global Cancer Statistics?[J].Cancer Commun (Lond),2019,39(1):22.
• [6] Zhang Y,Jiang Y,Zhang M,et al.Ganoderma sinense polysaccharide:an adjunctive drug used for cancer treatment[J].Prog Mol Biol Transl Sci,2019,163:165-177.
• [7] Zhao X,Zhou D,Liu Y,et al.Ganoderma lucidum polysaccharide inhibits prostate cancer cell migration via the protein arginine methyltransferase 6 signaling pathway[J].Mol Med Rep,2018,17(1):147-157.
• [8] Zolj S,Smith MP,Goines JC,et al.Antiproliferative effects of a triterpene-enriched extract from Lingzhi or Reishi medicinal mushroom,Ganoderma lucidum (Agaricomycetes),on human lung cancer cells[J].Int J Med Mushrooms,2018,20(12):1173-1183.
• [9] Acevedo-Díaz A,Ortiz-Soto G,Suárez-Arroyo IJ,et al.Ganoderma lucidum extract reduces the motility of breast cancer cells mediated by the RAC-lamellipodin axis[J].Nutrients,2019,11(5).
• [10] Ferreira IC,Heleno SA,Reis FS,et al.Chemical features of Ganoderma polysaccharides with antioxidant,antitumor and antimicrobial activities[J].Phytochemistry,2015,114:38-55.
• [11] Sohretoglu D,Huang S.Ganoderma lucidum polysaccharides as an anti-cancer agent[J].Anticancer Agents Med Chem,2018,18(5):667-674.
• [12] Ahmad MF.Ganoderma lucidum:a rational pharmacological approach to surmount cancer[J].J Ethnopharmacol,2020,260:113047.
• [13] Khan I,Huang G,Li XA,et al.Mushroom polysaccharides and Jiaogulan saponins exert cancer preventive effects by shaping the gut microbiota and microenvironment in ApcMin/+ mice[J].Pharmacol Res,2019,148:104448.
• [14] Luo J,Li T,Xie J,et al.Guar gum different from Ganoderma lucidum polysaccharide in alleviating colorectal cancer based on omics analysis[J].Food Funct,2020,11(1):572-584.
• [15] Dekker E,Rex DK.Advances in CRC prevention:screening and Surveillance[J].Gastroenterology,2018,154(7):1970-1984.
• [16] Bai JH,Xu J,Zhao J,et al.Ganoderma lucidum polysaccharide enzymatic hydrolysate suppresses the growth of human colon cancer cells via inducing apoptosis[J].Cell Transplant,2020,29:963689720931435.
• [17] Pan H,Wang Y,Na K,et al.Autophagic flux disruption contributes to Ganoderma lucidum polysaccharide-induced apoptosis in human colorectal cancer cells via MAPK/ERK activation[J].Cell Death Dis,2019,10(6):456.
• [18] Mizumoto Y,Yokoyama S,Matsuda K,et al.Modulation of capecitabine administration to improve continuity of adjuvant chemotherapy for patients with colorectal cancer:a phase II study[J].Mol Clin Oncol,2020,12(2):126-133.
• [19] Lu J,He R,Sun P,et al.Molecular mechanisms of bioactive polysaccharides from Ganoderma lucidum (Lingzhi),a review[J].Int J Biol Macromol,2020,150:765-774.