刘伟志;洪伟;李金龙;

• 1:辽宁中医药大学附属第三医院大肠外科
• 2:辽宁中医药大学附属第三医院电检科

摘要(Abstract)：

目的研究灵芝多糖在结直肠癌细胞中的调控作用。方法以结直肠癌细胞T84和DiFi作为研究对象,并以空白培养基和结直肠癌化疗药物卡培他滨分别作为空白对照和阳性对照。采用MTT法检测灵芝多糖(GLP)对T84和DiFi细胞增殖的影响,划痕愈合实验检测GLP对T84和DiFi细胞迁移能力的影响,Transwell实验检测GLP对T84和DiFi细胞侵袭能力的影响,流式细胞术检测GLP对T84和DiFi细胞凋亡率的影响。结果 MTT实验结果显示,灵芝多糖处理后,结直肠癌细胞的活力和增殖能力均显著低于空白组(P<0.05);划痕愈合实验和Transwell实验结果显示,灵芝多糖处理后,结直肠癌细胞的迁移、侵袭能力均显著低于空白组(P<0.05);流式细胞术检测细胞凋亡结果显示,灵芝多糖处理后结直肠癌细胞凋亡率显著高于空白组(P<0.05);GLP组与卡培他滨组差异无统计学意义(P>0.05)。结论灵芝多糖对结直肠癌细胞的增殖、迁移和侵袭能力均有良好的抑制作用,可促进结直肠癌细胞凋亡。

关键词(KeyWords)： 灵芝多糖;结直肠癌;卡培他滨

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 刘伟志;洪伟;李金龙;

DOI: 10.14053/j.cnki.ppcr.202108001

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