杨玲;邵锁;王常丽;任天舒;赵庆春;

• 1:沈阳军区总医院药剂科
• 2:沈阳药科大学药学院

摘要(Abstract)：

目的探索外周血代替肿瘤组织进行MTHFR C677T基因多态性检测的可行性。方法选取首诊为结直肠癌且行根治性手术的患者26例,术前未接受任何化学治疗,应用聚合酶链反应-限制性内切片段长度多态性(PCR-RFLP)方法检测MTHFR C677T的基因多态性,比较外周血及肿瘤组织中相关基因型的一致性。结果26例结直肠癌患者的肿瘤组织及外周血中MTHFR 677CT基因型、TT基因型、CC基因型一致的病例数分别为7、7、4例。肿瘤组织和外周血中MTHFR C677T的基因型一致率为69.2%。结论外周血中MTHFR C677T的基因多态性可以反映肿瘤组织中相关基因型的突变。

关键词(KeyWords)： 结直肠癌;肿瘤组织;外周血;MTHFR C677T

Abstract:

Keywords:

基金项目(Foundation): 辽宁省自然科学基金项目(201102244)

作者(Authors): 杨玲;邵锁;王常丽;任天舒;赵庆春;

DOI: 10.14053/j.cnki.ppcr.2013.10.038

参考文献(References)：

• [1]Jain VK,Hawkes EA,Cunningham D.Integration of biologic agents with cytotoxic chemotherapy in metastatic colorectal cancer[J].Clin Colorectal Cancer,2011,10(4):245-257.
• [2]Lucas AS,O'Neil BH,Goldberg RM.A decade of advances in cytotoxic chemotherapy for metastatic colorectal cancer[J].Clin Colorectal Cancer,2011,10(4):238-244.
• [3]Moghimi-Dehkordi B,Safaee A.An overview of colorectal cancer survival rates and prognosis in Asia[J].World J Gastrointest Oncol,2012,4(4):71-75.
• [4]Park DJ,Lenz HJ.Determinants of chemosensitivity in gastric cancer[J].Curr Opin Pharmacol,2006,6(4):337-344.
• [5]姚成云,黄新恩,黎超,等.在晚期非小细胞肺癌中XRCC1和XPD基因多态性的联合和铂类化疗的关系[J].徐州医学院学报,2010,30(6):391-396.
• [6]Sarbia M,Stahl M,Von Weyhern C,et a1.The prognostic significance of genetic polymorphisms(Methylenetetrahydrofolate Reductase C677T,Methionine Synthase A2756G,Thymidilate Synthase tandem repeat polymorphism)in multimodally treated oesophageal squamous cell carcinoma[J].Br J Cancer,2006,94(2):203-207.
• [7]唐金海,胡亦钦,秦建伟,等.亚甲基四氢叶酸还原酶和胸苷酸合成酶基因多态与乳腺癌化疗敏感性关系的研究[J].中华肿瘤防治杂志,2008,15(1):39-43.
• [8]Frosst P,Blom HJ,Milos R,et al.A candidate genetic risk factor for vascular disease:a common mutation in methylenetetrahydrofolate reductase[J].Nat Genet,1995,10(1):111-113.
• [9]Fernández-Peralta AM,Daimiel L,Nejda N,et a1.Association of polymorphisms MTHFR C677T and A1298C with risk of colorectal cancer,genetic and epigenetic characteristic of tumors,and response to chemotherapy[J].Int J Colorectal Dis,2010,25(2):141-151.
• [10]Cohen V,Panet-Raymond V,Sabbaghian N,et a1.Methylenetetrahydrofolate reductase polymovphism in advanced colorectal cancer:a novel genomie predictor of clinical response to fluompyfimidine-based chemotherapy[J].Clin Cancer Res,2003,9(6):1611-1615.